専門分野

• 分析化学
• タンパク質化学

担当科目

• 機器分析
• 分析化学
• 分析化学実習

メッセージ

• 一緒にがんばりましょう。

学歴

• 2013年 京都大学大学院理学研究科化学専攻博士後期課程修了

学位

• 博士 (理学)

主な職歴

• 2024年
• 姫路獨協大学 講師
• 2020年
• 姫路獨協大学 助教
• 2013年
• 姫路獨協大学 助手

所属学会

• 日本薬学会
• 日本化学会

論文・解説

原著論文

• Kenzo Aki, Emiko Okamura, “Real-Time 1H NMR Reveals Position and Sequence Dependences of Amino Acid Isomerization in Amyloid Beta Fragments in Situ”, Journal of Molecular Liquids, 364, 120050 (2022). Impact factor:6.0(2022), 6.633(2021), 6.165 (2020), 5.065 (2019) 4.561 (2018)
• Emiko Okamura, Kenzo Aki, “Real-time in-situ 1H NMR of reactions in peptide solution: preaggregation of amyloid-β fragments prior to fibril formation ” Pure and Applied Chemistry, 92, 1575-1583 (2020). 5-year Journal Impact Factor 2.2
• Kenzo Aki, Emiko Okamura,“Side-chain conformers to allow conversion from normal to isoaspartate in age-related proteins and peptides ” BBA-Proteins and Proteomics, 1868, 140483 (2020). Impact factor:3.2(2022), 4.125(2021), 3.036 (2020), 2.371 (2019) 2.540 (2018)
• Kenzo Aki, Emiko Okamura, “Isomerization of aspartyl residue in amyloid beta fragments: The kinetics by real-time 1H NMR under neutral and basic conditions” J. Soln. Chem., 49, 1293-1303 (2020). Impact factor:1.2(2022), 2.000(2021), 1.677(2020), 1.273(2019) 1.039(2018)
• Yuki Takechi-Haraya, Kenzo Aki, Yumi Tohyama, Yuichi. Harano, Toru Kawakami, Hiroyuki Saito, Emiko Okamura “Glycosaminoglycan Binding and Non-endocytic Membrane Translocation of Cell-permeable Octaarginine Monitored by Real Time In-cell NMR Spectroscopy” Pharmaceuticals, 10, 42 (2017).　5-year Journal Impact Factor: 4.9
• Kenzo Aki, Emiko Okamura. “Kinetics of the competitive reactions of isomerization and peptide bond cleavage at L-α- and D-β-aspartyl residues in an αA-crystallin fragment”. J. Pept.Sci., 23, 28-37 (2017). Impact factor: 2.1(2022), 2.408 (2021), 1.905 (2020), 1.877 (2019) 2.081 (2018)
• Kenzo Aki, Emiko Okamura. “D-β-aspartyl residue exhibiting uncommon high resistance to spontaneous peptide bond cleavage.” Sci. Rep., 6, 21594 (2016). Impact factor: 4,6(2022), 4.996 (2021), 4.379 (2020), 3.998 (2019) 4.011 (2018)
• Kenzo Aki, Emiko Okamura. “Staggered side-chain conformers of aspartyl residues prerequisite to transformation from L-α- to D-β-aspartate 58 in human-lens αA-crystallin fragment.” Biophysical Chemistry 196: 10-15 (2015) Impact factor: 3.8(2022), 3.628 (2021), 2.352 (2020), 1.995 (2019) 1.745 (2018)
• Emiko Okamura, Yuki Takechi, Kenzo Aki. “Uptake of Sevoflurane Limited by the Presence of Cholesterol in the Lipid Bilayer Membrane: A Multinuclear Nuclear Magnetic Resonance Study.” Journal of Oleo Science 63(11):1149-1157 (2014) Impact Factor: 1.5 (2022), 1.628 (2021), 1.601 (2020), 1.304 (2019) 1.208 (2018)
• Kenzo Aki,N orihiko Fujii, Noriko Fujii. “Kinetics of isomerization and inversion of aspartate 58 of αA-crystallin　peptide mimics under physiological conditions” PLoS ONE （PlOS ONE PONE-D-12-35860R1 10.1371/jurnal.pone.0058515 (2013) Impact factor: 3.7 (2022), 3.752 (2021), 3.240 (2020), 2.740 (2019) 2.776 (2018)
• Kenzo Aki, Norihiko Fujii, Noriko Fujii. “Characterization of an antibody that recognizes peptides containing D-β-aspartyl residues.” Molecular Vision 18, 996-1003 (2012) Impact factor: 2.2(2022), 2.711 (2021), 2.367 (2020), 2.202 (2019) 2.174 (2018)
• Yuhei Mori, Kenzo Aki, Katsunori Kuge, Natsuko Yamanaka, Shingo Tajima, Yuichi Kaji, Ryoji Nagai, Hanako Yoshii, Naoki Yamamoto, Norihiko Fujii, Masami Watanabe, Tadatoshi Kinouchi, Noriko Fujii. UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of Ne-carboxymethyl lysine (CML) in keratin of skin. J.Chromatogr. B 879, 3303-3309 (2011). Impact factor: 3.0(2022), 3.318 (2021), 3.205 (2020), 3.004 (2019) 2.813 (2018)

総説・解説

• Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki, Hiroaki Sakaue. “The mirror of life as a molecular index of aging.” Biochimica et Biophysica Acta 1866, 840-847 (2018).
• Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki, Hiroaki Sakaue. “D-Amino acid residues in proteins related to aging and age-related diseases and a new analysis of the isomers in proteins.” In “D-Amino acids: Physiology, Metabolism, and Application” edited by Yoshimura T, Nishikawa T and Homma H. Springer, pp 241- 254 (2016) ISBN: 978-4-431- 56075-3
• Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki. “Isomerization of aspartyl residues in crystallins and its influence upon cataract.” Biochimica et Biophysica Acta 1860, 183-191 (2016).
• Noriko Fujii, Kenzo Aki, Norhiko Fujii. “D-amino-acid-containing proteins in living body. ” In“The Soai Reaction and Related Topic” edited by Gyula Pály, Claudia Zucchi, Luciano Caglioti. Accademia Nazionale di Scienze Lettere e Arti Modena, Italy. 　Chapter 2, pp35-52 (2012)

口頭発表・ポスター

国際会議

• Kenzo Aki, Emiko Okamura “Kinetics of Peptide Bond Cleavage and Isomerization at Aspartyl Residue Using Real-Time NMR Spectroscopy” International Conference of D-Amino Acid Research　2019 9/10-13 Tokyo, Japan
• Kenzo Aki, Emiko Okamura “The Kinetics of Amino Acid Isomerization in of Amyloid Beta Fragments Quantified by Real-Time 1H-NMR” The 36th International Conference on Solution Chemistry, 2019 8/4-8 Xining, China. 「第36回溶液化学国際会議 (IUPACなど共催)」において、最優秀ポスター賞を受賞
• Yuhei Mori, Kenzo Aki, Tadatoshi Kinouchi, Takehiro Kawaguchi, Masashi kida, Hiroaki Sakaue and Noriko Fujii. “Simultaneous racemization and glycation of amino acid residues in proteins of UV B irradiated skin” International Conference of D-Amino Acid Research, 2009 7/1-4 Awaji, Japan.

招待講演

• 安岐　健三、岡村　恵美子　“アスパラギン酸の異性化及び切断反応における複数残基同時観測と速度論解析～リアルタイムNMRを用いて～” 第10回 タンパク質の異常凝集とその防御・修復機構に関する研究会　大阪(2018) 12/11-12
• 安岐　健三、藤井　智彦、藤井　紀子　“αAクリスタリンの部分ペプチドを用いたAsp異性化シミュレーション”　第86回日本生化学会大会　横浜 (2013) 9/11-13

受賞歴

• 「第36回溶液化学国際会議 (IUPACなど共催)」において、最優秀ポスター賞を受賞